| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062673 |
| Chromosome: | chromosome 2 |
| Location: | 5902087 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117600 | 5'UTR | ||
| Cre02.g117650 | GT90F2,GT90-2 | (1 of 4) PTHR12203//PTHR12203:SF35 - KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // SUBFAMILY NOT NAMED; Glycosyltransferase GT90 family protein 2 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGCGGCGGATTGGCGCTACCCAGCAGGTCCCGTCAGGTCCCGTTCC |
| Internal bar code: | TAGTGTTTACTGCAAATGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4598 |
| LEAP-Seq percent confirming: | 74.4444 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTCTGTGACGCAAGCTG |
| Suggested primer 2: | TGACTGGCTTCACGTACCAC |