Insertion junction: CLIP2.062683_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062683
Chromosome:chromosome 6
Location:1706572
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g262500 (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACGCCAGCACTCACTCCGCCAGCATCAGCAGCACGCTGC
Internal bar code:AGACAAGGTATAAGTAACTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3159
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGACCTGGACATCCTCC
Suggested primer 2:CGGATGTCACGTCACATGGA