Insertion junction: CLIP2.062690_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.062690
Chromosome:chromosome 6
Location:3971336
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278204 (1 of 4) PTHR10334//PTHR10334:SF224 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCACATGTTCTCAAACCAAGCACCAACGCGCCACAACTCAGCGCAAAC
Internal bar code:TCGCGATCAACTTTTGGTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3539
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:101
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:101

Suggested primers for confirmation by PCR

Suggested primer 1:TCACACGTGTTCCGGTACTG
Suggested primer 2:CATGAATCTGTGCGAGCTGC