Insertion junction: CLIP2.062701_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062701
Chromosome:chromosome 9
Location:4161090
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399626 (1 of 1) K09919 - hypothetical protein (K09919) 3'UTR
Cre09.g399663 FXL7,FXL4 FixL-like PAS domain protein; (1 of 11) PF13426 - PAS domain (PAS_9) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGATCACCAGATCACCAGAGGTGTCCCATACGGGTGCACCTGCCCGTCA
Internal bar code:GGCGGTCCTCCTTGGCGCGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:355
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACTCGCATGCCTCTCTT
Suggested primer 2:GCTCGATTTGTGGTCGCATC