| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062703 |
| Chromosome: | chromosome 14 |
| Location: | 4122716 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633850 | (1 of 1) PF14768 - Replication protein A interacting C-terminal (RPA_interact_C) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATATGTGCGACCAGCCTGGCGAGCCTGCAGGTGCTGCTGCCAGACTTGG |
| Internal bar code: | TTGCGCAAGCGATGTGGCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 788 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGGAGCACAAGGGGAAG |
| Suggested primer 2: | ACTCCCCACCCTCCAGTATC |