| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062712 |
| Chromosome: | chromosome 6 |
| Location: | 7267122 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298950 | (1 of 2) PF10343 - Potential Queuosine, Q, salvage protein family (Q_salvage) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGTGATCATATCCACAAGCACTATGCTTGAGCTGTATAGAAAGCTTT |
| Internal bar code: | GACGCGCCTAAGTTTAAAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2257 |
| LEAP-Seq percent confirming: | 90.6977 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGGAGAGTCACCGAGCA |
| Suggested primer 2: | GTTTGGTCTTTGGACGGTGC |