| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062716 |
| Chromosome: | chromosome 3 |
| Location: | 3369253 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166350 | (1 of 1) IPR017849//IPR017850 - Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCTGGCGACCGCCCACGCCATGGCCAGGCTGGTGTGCTCGGGTTGTG |
| Internal bar code: | CCAGTGCTCAAAGGCCCGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3311 |
| LEAP-Seq percent confirming: | 39.1304 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAACGGAAGACCCCCTC |
| Suggested primer 2: | TGTGGTACTTTGCTCCCGTC |