| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062719 |
| Chromosome: | chromosome 16 |
| Location: | 4163232 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670500 | (1 of 7) PF02190 - ATP-dependent protease La (LON) substrate-binding domain (LON_substr_bdg) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCGGGACCAAATGCGGAATTGCGGTTCTTATCAGCTGCAGGGGTTCT |
| Internal bar code: | TCTTTTCAGTTTTTAAAAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2501 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACACGCAGGAAGACAGA |
| Suggested primer 2: | CTGCAGGGATTAGTGGCCAA |