| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.062726 |
| Chromosome: | chromosome 12 |
| Location: | 1569092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487400 | (1 of 2) PF03556//PF14555 - Cullin binding (Cullin_binding) // UBA-like domain (UBA_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTTGGGGCGTCTGAACTACATACGATTTGGACACTCCGCCATCCAAC |
| Internal bar code: | AAAACGTGGGCCGTGCGAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1426 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGTGCGTGGTTGTGAAG |
| Suggested primer 2: | CACCTTGTGTCTGTTGCGTG |