| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062762 |
| Chromosome: | chromosome 16 |
| Location: | 2023236 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656851 | (1 of 2) PF07856 - Mediator of CRAC channel activity (Orai-1) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTTTTGAGGGGGTAGTTGTAGAGCATACAAGGGGCTGTGTAAGGTCT |
| Internal bar code: | TGTGTGTGCTAACGATGTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2603 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGTGGAGAGCACTGCAG |
| Suggested primer 2: | CAGGTACTCTGCCTCCTCCT |