| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062763 |
| Chromosome: | chromosome 3 |
| Location: | 1243505 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149700 | (1 of 1) PF14518 - Iron-containing redox enzyme (Haem_oxygenas_2) | 5'UTR | |
| Cre03.g149750 | DMA2 | (1 of 2) 2.1.1.297 - Peptide chain release factor N(5)-glutamine methyltransferase / N(5)-glutamine S-adenosyl-L-methionine dependent methyltransferase; Putative DNA methylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCGTGCGCCGGCGCGGCGCAACGAGCTGCCGTTGAGACCGCTGCTGG |
| Internal bar code: | CAAAGGACTCACCTCCCACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1969 |
| LEAP-Seq percent confirming: | 22.7273 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTTGCAAGCCCGTTTTC |
| Suggested primer 2: | GATTCCTCCGCACACTGGAA |