Insertion junction: CLIP2.062782_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062782
Chromosome:chromosome 16
Location:4163122
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670500 (1 of 7) PF02190 - ATP-dependent protease La (LON) substrate-binding domain (LON_substr_bdg) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCGTGCGTTGCATGTTTGGCTGTGAACTTGATTGCGCGAGAGAGACAG
Internal bar code:TATTAGTCGAGCGCGTGTACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2585
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GACACACGCAGGAAGACAGA
Suggested primer 2:CTGCAGGGATTAGTGGCCAA