Insertion junction: CLIP2.062790_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.062790
Chromosome:chromosome 3
Location:8414717
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g213425 COX23 (1 of 9) IPR009069 - Cysteine alpha-hairpin motif superfamily; Cytochrome c oxidase assembly protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGCACACACCTGCCACGCCACCGTAGCCCCATAAGACCGCCTGCGCC
Internal bar code:AAAGAAATGCAACGCAATCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2581
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGTCGACACTGAAGGTT
Suggested primer 2:GCAGAGCGTGTAAATGAGCG