| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062798 |
| Chromosome: | chromosome 12 |
| Location: | 4402244 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519850 | (1 of 1) K11549 - kinetochore protein Spc24, animal type (SPBC24, SPC24) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCGTCACAGCTCTCTGCCATGCACCACTTCCTGCTTCCGCATCCTCT |
| Internal bar code: | CTTTTAGGCGTTGCTGTGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGAGAAGCAGCGGCAAA |
| Suggested primer 2: | TCAAGAGCTCATGCAGGCAA |