| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.062799 |
| Chromosome: | chromosome 6 |
| Location: | 334721 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251100 | NMT1 | (1 of 1) 2.3.1.97 - Glycylpeptide N-tetradecanoyltransferase / Peptide N-tetradecanoyltransferase; N-myristoyl transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTAGGGCTCACGCCCACGTACAACCCCGATCCCCAGATGTCAGGCCC |
| Internal bar code: | TATTAAATAGGAGTGCGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2307 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGTTCCAGAACATCGA |
| Suggested primer 2: | ATTCCATGCTCACCCAGTGG |