Insertion junction: CLIP2.062819_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062819
Chromosome:chromosome 4
Location:34920
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g213985 (1 of 62) PF00226 - DnaJ domain (DnaJ) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTGTCAGTGCACTCGGCACTTCCAAGGCCAGGAGCCAACACCAGCAAG
Internal bar code:CAACACTTAGGTCATAAGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2420
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTCTTGCCGCTCTAGT
Suggested primer 2:TTCGAGTCAGTGGCAAGGTC