| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.062826 |
| Chromosome: | chromosome 4 |
| Location: | 510353 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217929 | (1 of 1) 1.8.2.1//1.8.3.1 - Sulfite dehydrogenase // Sulfite oxidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGAAAGATAAGTAGTGCATGCTGGATCCTGAAGCTGTCCATCGAGGC |
| Internal bar code: | TTCTCCGGCATGAGGGCTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGAGCGCCGTTATAGAA |
| Suggested primer 2: | TGCATCTCGATGAACTGCGT |