| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062833 |
| Chromosome: | chromosome 1 |
| Location: | 6690087 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047700 | CYN40 | Cyclophilin 40; (1 of 2) K05864 - peptidyl-prolyl isomerase D [EC:5.2.1.8] (PPID, CYPD) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGAGATTCCACCGTGCCTAGCAAGTTCGCTGCCGGATCACGTGCGGC |
| Internal bar code: | GTAAGGCCCCTGTGATATACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4326 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGACCGTTGGCCTTCTTC |
| Suggested primer 2: | CGCCGATGCTGATGTCAAAG |