Insertion junction: CLIP2.062838_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.062838
Chromosome:chromosome 7
Location:6270025
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g356700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAGCGCCCGGCTGCTGCTGACACCACCCCTGCTGCGAGGCCTGCTGC
Internal bar code:CTTTCTCCGGGGTCATAGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2246
LEAP-Seq percent confirming:1.88679
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:52
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCACACCACCAGCAACAA
Suggested primer 2:GGGTAGGAGGTCGAGACTGT