| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062839 |
| Chromosome: | chromosome 9 |
| Location: | 4853917 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402849 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATAGGAGCCCTGTGTGGTGGTAGCTGTGGTGGTAGCTGTGGTGGTGG |
| Internal bar code: | ATGGGCCAACGGGGAGGCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGCCCTCAGGGAATGGG |
| Suggested primer 2: | ATGTGATTGGGAGTGAGGCG |