| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062844 |
| Chromosome: | chromosome 2 |
| Location: | 5366755 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113500 | (1 of 1) IPR001322//IPR014867 - Lamin Tail Domain // Spore coat protein CotH | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAAAGGACACGTTGGCATACTGCCTGCGGGAAGTGGTCAGATTGTGG |
| Internal bar code: | GGGGTAGTTTTTAACGATGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 63.3333 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGTGGGACGAACAGAAG |
| Suggested primer 2: | CCAGTTCACGACGTACACCA |