Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.062856 |
Chromosome: | chromosome 12 |
Location: | 8733401 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g547400 | (1 of 5) PF12681 - Glyoxalase-like domain (Glyoxalase_2) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGATCATGCCGTCGCCACGTCGACAACCTTACACCGACCCTTACCAT |
Internal bar code: | TTGGCTGGAAAATCCTATCATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3080 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 73 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTTGCTTGCCTGTTTCTCC |
Suggested primer 2: | TCGCTTGGGAATTCGGACAA |