| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062886 |
| Chromosome: | chromosome 14 |
| Location: | 1654045 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618700 | uS7m,MRPS7 | Mitochondrial ribosomal protein S7; (1 of 2) K02992 - small subunit ribosomal protein S7 (RP-S7, MRPS7, rpsG) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGGAAATGCTTTATGCATTGTAATCGACTATCTATACAACTGCAGAC |
| Internal bar code: | GCACTCAACAACGTGCATAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1833 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGGCGTGAAGAGGTCTA |
| Suggested primer 2: | CTTGTTGCGCTTGTTCACGA |