Insertion junction: CLIP2.062890_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.062890
Chromosome:chromosome 15
Location:440878
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g635700 MAPKKK12 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Mitogen-Activated Protein Kinase Kinase Kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGACTGGGCCCTCGCCCCCCCCCCACGCCACACGCACACGCACACAAA
Internal bar code:CTATAGATTTTCTTGCAATATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1414
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGCCCTCTTTGCTTGGTG
Suggested primer 2:GCAACCCCATCGTCTACACA