Insertion junction: CLIP2.062900_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.062900
Chromosome:chromosome 4
Location:2128426
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g219787 (1 of 1) 2.5.1.74 - 1,4-dihydroxy-2-naphthoate polyprenyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGCTTCTGTGGTCCTCTCGAACACACCACGTCGCTTTGAGCCCTCCT
Internal bar code:CGTGGTGAAGTGTAGTAGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:281
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTTCACCAGATAGCGGGC
Suggested primer 2:GAAGGGGTTCTGCATTTCGC