| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.062901 |
| Chromosome: | chromosome 3 |
| Location: | 6424685 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192950 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCATCTTCCTGTCACAAATTACAGGCATTTTGCTGGACCAGCTGTTCT |
| Internal bar code: | TTTATGATACTTAATTTTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2626 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACATCTCCGGCGCTGATTC |
| Suggested primer 2: | CAAAGTCTCGCCCCAGACAT |