| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062905 |
| Chromosome: | chromosome 16 |
| Location: | 1514468 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCCGAGGTCAAGGAATGTCAAGGGACTTTAACGAGGTCCGGCCCCCT |
| Internal bar code: | GGACTCAATACGGCCTAATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5006 |
| LEAP-Seq percent confirming: | 99.0196 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAGCTTATGCATTCCCC |
| Suggested primer 2: | GCGCTTCTGCTTGTGTGAAA |