| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.062913 |
| Chromosome: | chromosome 10 |
| Location: | 1487740 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428600 | (1 of 13) PF00646 - F-box domain (F-box) | 3'UTR | |
| Cre10.g428650 | CDPKK1,CDPKKK1 | (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Calcium/calmodulin-dependent protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCAAGTCAAGCGCGGCGAAACTTCTGGTGTGAACTTCAGTCCAGTT |
| Internal bar code: | AGTAAGGATAAAGTAGTAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2115 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGGAGATGGAGGCACGG |
| Suggested primer 2: | GCCTTGGTGGTCCATGATGA |