Insertion junction: CLIP2.062921_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.062921
Chromosome:chromosome 4
Location:2976172
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224867 (1 of 34) IPR001878 - Zinc finger, CCHC-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGCCTTGTGTATCGGCACCGCATACCGCATGTGATTCGGTGCCTTGT
Internal bar code:ATCTATTAGATTACGCGCGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1861
LEAP-Seq percent confirming:67.5325
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACGCCATCAACAACAGT
Suggested primer 2:GCTACCCCTTCGACGAGAAC