Insertion junction: CLIP2.062924_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.062924
Chromosome:chromosome 6
Location:7359094
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g299900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGTGTAGAAATACGATAAGAAGTTAGTCCTGACATTGTACAGCGCGG
Internal bar code:CCCGTTGCTATGGCTATACATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2391
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:AATATCGAGCGTGACGGACC
Suggested primer 2:CAGTCGGAAGCACAAACAGC