| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062944 |
| Chromosome: | chromosome 8 |
| Location: | 1564827 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365400 | PRPL31 | Chloroplast ribosomal protein L31; (1 of 1) K02909 - large subunit ribosomal protein L31 (RP-L31, rpmE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCACATCAAGCGCCGCACGGGTCGGCATGTCGGCATGCCGCCTCGCC |
| Internal bar code: | AAACTCGGAGACATTTTTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2143 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGGTGTGGAGGTCATGA |
| Suggested primer 2: | TTGCCCTCACTTGAATCCCC |