Insertion junction: CLIP2.062960_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.062960
Chromosome:chromosome 17
Location:4317131
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g730100 RSE1,RSEP1 (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGTCTCCCAGACGTGCCATGGTGTTACATGTGCGTTCGGCAGCCTGT
Internal bar code:ACCTATACTATGGCCGCCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2351
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGGGGATGGTAGGCATT
Suggested primer 2:AGTCCTAATGCGCCACAACA