| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062966 |
| Chromosome: | chromosome 9 |
| Location: | 3341896 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395473 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGTCATCGCCACACGTGTCCTCAGCCCAAGCATCTTCAGCCGTGCGC |
| Internal bar code: | CATTTATACAAGTGCGACCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCGCAGAGGATTCCTAG |
| Suggested primer 2: | CGCTGCCTAAATGAATGGCC |