| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.062969 |
| Chromosome: | chromosome 2 |
| Location: | 6524588 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389000 | (1 of 1) PTHR23012:SF61 - AT07234P-RELATED | 3'UTR | |
| Cre09.g389050 | CNX5,MAW4 | (1 of 2) PTHR16861//PTHR16861:SF4 - GLYCOPROTEIN 38 // PODOPLANIN; Membrane-associated hydroxyproline-rich glycoprotein 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGTGTGCAGAGTATGGAGGATAGGTTGATGCTGTCCTCCCCAGCCG |
| Internal bar code: | TCTGGGTACTTAATCTCCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1039 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCGCTCCATCCAGACCTA |
| Suggested primer 2: | CCCAGGCTTACGCTGTTGTA |