Insertion junction: CLIP2.062979_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062979
Chromosome:chromosome 8
Location:1628148
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g365800 (1 of 1) IPR000104//IPR001077//IPR016461//IPR029063 - Antifreeze protein, type I // O-methyltransferase, family 2 // O-methyltransferase COMT-type // S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAATGGTCAAAGGATCGGCGGGGGAGGAGAGAGCGGGGGAGGGGAGGGC
Internal bar code:GTTTGGAAGTCCAACATTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:628
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCCAAGCCATCTACTGC
Suggested primer 2:CATGTGTGTGGCAGTGTGTG