Insertion junction: CLIP2.062987_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062987
Chromosome:chromosome 6
Location:4672707
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278600 (1 of 1) PF11221 - Subunit 21 of Mediator complex (Med21) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGAAGCGTTGTGTCTGCAATCCGCTCTCACTCCCCACTGCGCTCCCA
Internal bar code:GGAAGGAGTGTTCAACAATACG

Confirmation - LEAP-Seq

LEAP-Seq distance:5062
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCATCGCTTGGCATCCTT
Suggested primer 2:CGTTGCATAACTCGCACTCG