| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062996 |
| Chromosome: | chromosome 10 |
| Location: | 1628003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429350 | (1 of 6) 3.4.22.69 - SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCTAGCACTTGCTGCATCCACGCTCCTCGGTCTGGCGCTCTTACAG |
| Internal bar code: | TCATGGCTTCACTTAGTAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2364 |
| LEAP-Seq percent confirming: | 93.9024 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGAGAGCTCGGACGAAG |
| Suggested primer 2: | GCTGCAACGGCCATAACAAA |