Insertion junction: CLIP2.062996_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062996
Chromosome:chromosome 10
Location:1628006
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g429350 (1 of 6) 3.4.22.69 - SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTGCAATTGTTGGGGTAGTTGGGGGCGTGGCGATGTGAGCTTCCTTGG
Internal bar code:CGCAAGCTGGAAATCCCCCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3298
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCAACGGCCATAACAAA
Suggested primer 2:GGATGAGAGCTCGGACGAAG