| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.062999 |
| Chromosome: | chromosome 12 |
| Location: | 4457222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520173 | (1 of 1) PTHR10887//PTHR10887:SF340 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED | 5'UTR | |
| Cre12.g520200 | CPL8,PPO5 | (1 of 1) PTHR13343//PTHR13343:SF16 - CREG1 PROTEIN // SUBFAMILY NOT NAMED; Conserved in the Plant Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGCGTTGCGTGCTGAGTTAGTGTAGGGCATGCACGGCCTTCTTATCG |
| Internal bar code: | TGAGGAGTAGTAACTCATGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5460 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCATCCATGACAGTTGG |
| Suggested primer 2: | ACAGCAGCATGAGGAAGTCC |