Insertion junction: CLIP2.063010_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.063010
Chromosome:chromosome 11
Location:2328718
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095134 (1 of 2) 2.1.1.9 - Thiol S-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATCTCAAACTATGCATCATTGCCACGACGCTGTACCTTATACCTCTG
Internal bar code:CTTGTAAAAGGTAGGGCTCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1145
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGAGTAAGCCACCAGAGA
Suggested primer 2:CTGTCACGTCCAACCTCGAA