| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.063017 |
| Chromosome: | chromosome 5 |
| Location: | 2711345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGACTGCCATCTACTCTCCGCAGTAACCTTCGAATTGTAGATCAACA |
| Internal bar code: | AACCTGTTAATACGTATTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2354 |
| LEAP-Seq percent confirming: | 93.4783 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACGTGCGACTGAAGGT |
| Suggested primer 2: | GTGGAGGTTTGTAGAGGGGC |