Insertion junction: CLIP2.063035_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063035
Chromosome:chromosome 9
Location:2418010
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393650 (1 of 1) PF00069//PF01590 - Protein kinase domain (Pkinase) // GAF domain (GAF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGACTCCTCGGCGCCACGCCACATTTACCACCCGCAACCCCAGCGAGC
Internal bar code:CGGAGGGGTATAGAAATTTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2665
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTGGTTTCGACACG
Suggested primer 2:CCAGCAACAACGCTATCTGC