Insertion junction: CLIP2.063047_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.063047
Chromosome:chromosome 7
Location:1123673
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g320650 PIGV,PIGV1 (1 of 1) K07542 - phosphatidylinositol glycan, class V (PIGV); alpha-(1-6)-Mannosyltransferase II 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAAGGATGTCACACGAATTGGCGCGAAAGGACTGCGAACATGTGAAGCG
Internal bar code:CTAATCGTCAGGTGAACTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:3301
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGGTACATGGCCCACCTG
Suggested primer 2:CCAGGAGAAGTCGGTGTCAC