| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.063053 |
| Chromosome: | chromosome 17 |
| Location: | 3516300 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724450 | RAA10 | component psaA trans-splicing complex I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCATGGGAGCGTGCCGTCGTGCCGGTTATGTTGAACCCTCTCCCCAG |
| Internal bar code: | GTGGAGTTTTTTCAGGATTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGAGTACTGCGTGGTGTC |
| Suggested primer 2: | AGTGTTTAGCGAGCCGTTCA |