Insertion junction: CLIP2.063064_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063064
Chromosome:chromosome 12
Location:2922914
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCTCGCGGCTATCCCTGCCATCCTATCTCCCTGTGCCTAGGTGCTCT
Internal bar code:GCAACTCGCCATAAAGCTCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:2030
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGTGTGGCAATGTGTTG
Suggested primer 2:ATTCCTCGCTGCATACTCCG