| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.063067 |
| Chromosome: | chromosome 16 |
| Location: | 1039169 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649250 | (1 of 4) PF13911 - AhpC/TSA antioxidant enzyme (AhpC-TSA_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATTGTACAGCCCGCTAACCTAACCACAGCTTACAGGCCCGCGCTTGC |
| Internal bar code: | CGTGGCATGGGTTAGTTAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1392 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCTACATGTTCCAAGCC |
| Suggested primer 2: | GGTAAGGGCCTAAGGGGGTA |