| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.063079 |
| Chromosome: | chromosome 5 |
| Location: | 2294377 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238150 | (1 of 1) PF07646 - Kelch motif (Kelch_2) | 3'UTR | |
| Cre05.g238200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGCCGAGGGCAGCAGTGCTCCGCCCTATGCCTTGCCGTTTCAGGCCG |
| Internal bar code: | ACGACTAATTTATTACAAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 992 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCTCAACATGGTTCCCA |
| Suggested primer 2: | GATTCTGGCTGGTGCTGACT |