| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063090 |
| Chromosome: | chromosome 7 |
| Location: | 1168741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321050 | (1 of 1) PTHR11772//PTHR11772:SF3 - ASPARAGINE SYNTHETASE // ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1 | 3'UTR | |
| Cre07.g321100 | (1 of 1) PTHR10196:SF67 - SEDOHEPTULOKINASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGTTGATGGGCAGTGCCCCGCAGGCACGCAGGCCTGGACTTCGCTGT |
| Internal bar code: | AATTCTACTCAAATTAGCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3597 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCATGGATGATAGGTGCT |
| Suggested primer 2: | GGCTCTAGCGGCTTTCTAGG |