| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063097 |
| Chromosome: | chromosome 17 |
| Location: | 5381309 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738450 | (1 of 5) 2.7.11.7 - [Myosin heavy-chain] kinase / Myosin II heavy-chain kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACGACTAGGCCTGGTGGAAATGGACCTGCGACAAGCCGTTGGTGGGC |
| Internal bar code: | TATTGGTAGGATGCTCTTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1853 |
| LEAP-Seq percent confirming: | 79.1667 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTGACGTCCTTTGAGCC |
| Suggested primer 2: | GTGAGCAACATCAGAGGGCT |