Insertion junction: CLIP2.063109_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.063109
Chromosome:chromosome 3
Location:97626
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g143787 5'UTR
Cre03.g800279 (1 of 1) PF00059//PF13229 - Lectin C-type domain (Lectin_C) // Right handed beta helix region (Beta_helix) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGAAAGCCTGCTACGGTAAGCTCGCTTCCAATCTTCATCCTGTCTGCA
Internal bar code:GGTCAACGCAAAGCGCGTCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:4768
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCCCCGTTTGAGCCAAAC
Suggested primer 2:CGCTGACGCTGAATAAAGCC